Serological Investigation of Bluetongue Virus Infection by Serum Neutralization Test and Elisa in Sheep and Goats
نویسندگان
چکیده
Altogether 562 sheep and goat blood serum samples were collected from livestock located in Konya, Burdur, and their environs in Turkey. The samples were tested for bluetongue virus antibodies by ELISA and serum neutralization test (SNT). Fifty-four (17.1%) out of 315 sheep serum samples from Konya and 1 (1.5%) out of 66 sheep serum samples from Burdur were detected as seropositive. Seventy-three (60%) out of 121 goat serum samples from Konya and 36 (60%) out of 60 goat serum sample from Burdur were found positive by ELISA. Fifty-three (16.8%) out of 315 sheep serum samples from Konya and none of 66 sheep serum samples from Burdur were detected to be seropositive. Sixtyfive (53.7%) out of 121 goat serum samples from Konya and 32 (53.3%) out of 60 goat serum samples from Burdur were found as positive by SNT. The results demonstrate that ELISA is more sensitive than SNT.
منابع مشابه
A Serological and Molecular study on Toxoplasma gondii infection in sheep and goat in Tabriz
Toxoplasmosis is an important zoonotic diseases in human and animals. The disease caused by the protozoan Toxoplasma gondii. To determine the infection rate of toxoplasmosis in sheep and goats in east Azarbaijan province of Iran a total of 186 sera, 13 fetal brains, 13 cotyledons and 34 whole blood samples were collected from sheep and goats in Tabriz abattoir during the year 2010. Serum sample...
متن کاملSerological and Molecular Detection of Akabane Virus in Iran
The present study was conducted on sixty healthy female domestic ruminants that suffered from still births or abortion, to find sero-positive cases and also to determine the probable role of uterine tissue in diagnosis of akabane viral infection and viral persistency. Because of this, a serological test (ELISA) and molecular (RT-PCR) diagnostic methods were used to find the seropositive cases a...
متن کاملA Serological Survey on Antibodies against Akabane Virus in Sheep in Southwest of Iran
Abstract Background and Aims: Akabane disease causes epidemics of abortions, stillbirths and congenital malformations manifested as arthrogryposis and hydranencephaly or microanencephaly in sheep, goats and cattle. Akabane virus replicates in arthropods and is transmitted by either mosquitoes or midges. Outbreaks of the Akabane virus have been reported from many countries in Southeast Asia...
متن کاملSerologic evidence of bluetongue infection in onehumped camels (Camelus dromedarius) in Kerman province, Iran
Herein, we presented the first report on bluetongue (BT) disease in 10 pregnant camels in a herd fromKerman province, Iran. All sera samples were tested serologically (AGID, C-ELISA). We also used the Razi-BK cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ECE) to culture and isolate the BT virus. Efforts to culture and isolation of...
متن کاملSerological differentiation of five bluetongue virus serotypes in indirect ELISA.
The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific f...
متن کامل